Published October 1986
| Version v1
Journal article
OH-induced free radicals in uridine studied by a method combining ESR, spin-trapping, and liquid chromatography
Description
Free radicals produced by the reactions of OH radicals with uridine were investigated by a method combining ESR, spin-trapping, and liquid chromatography. A N2O-saturated aqueous solution of uridine, containing 2-methyl-2-nitrosopropane as a spin-trap, was X-irradiated and the resulting spin-adducts were separated by gel permeation chromatography and reverse-phase HPLC. ESR and uv-absorbance spectra obtained from the separated spin-adducts show that 5-yl and 6-yl radicals are produced by OH addition to the 5,6 double bond of the base moiety. It is also shown that radicals due to H abstraction from the sugar moiety at the C-4' and C-5' positions are produced
Additional details
Publishing Information
- Journal Title
- Radiat. Res.
- Journal Issue
- no.1
- Series
- Radiat. Res.
- ISSN
- 0033-7587
- CODEN
- RAREA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18035713
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ADDUCTS; CHEMICAL RADIATION EFFECTS; CHROMATOGRAPHY; ELECTRON SPIN RESONANCE; HYDROXYL RADICALS; NITROSO COMPOUNDS; URIDINE; X RADIATION
- Descriptors DEC
- AZINES; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; IONIZING RADIATIONS; MAGNETIC RESONANCE; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; RADICALS; RESONANCE; RIBOSIDES; SEPARATION PROCESSES; URACILS