Published February 1975 | Version v1
Journal article

Measurement of human dopamine-β-hydroxylase in serum homologous radioimmunoassay

  • 1. Hoffmann-La-Roche, Inc., Nutley, NJ

Description

Human serum dopamine-beta-hydroxylase [3,4-dihydroxyphenylethylamine, ascorbate: oxygen oxidoreducatse (beta-hydroxylating), EC 1.14.17.1] levels have been determined in 52 samples with a newly devised radioimmunoassay that utilizes purified human enzyme and its specific antiserum. The values obtained were in excellent agreement (correlation coefficient r equals 0.98) with those obtained by measuring enzymatic activity in the same sera. Either assay technique, enzymatic or homologous radioimmunoassay, can be used for measurement of the enzyme in normal human serum.

Additional details

Additional titles

Augmented title (English)
"1"2"5I tracer technique

Identifiers

Publishing Information

Journal Title
Proceedings of the National Academy of Sciences
Journal Volume
72
Journal Issue
2
Series
Proc. Natl. Acad. Sci. U.S.A.
Journal Page Range
750-752
ISSN
0027-8424

Optional Information

Notes
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