Chromosome aberrations induced in human lymphocytes by U-235 fission neutrons. Pt. 3. Evaluation of the effect of the induced α and β activity on the chromosomal aberration yield
Creators
- 1. J. Stefan Inst., Ljubljana (Slovenia)
- 2. Inst. of Medical Research and Occupational Health, Univ. of Zagreb (Croatia)
- 3. Inst. of Oncology, Ljubljana (Slovenia)
Description
Aim: Further experiments were performed to explain a difference in chromosomal aberration yield found between samples cultivated immediately after fission neutron irradiation and samples which were cultivated with 96 h delay after irradiation. Material and Method: Human peripheral blood samples were irradiated in mixed fission neutron/gamma field (1800 s) and biological effect assessed in the mean of analysis of unstable chromosome aberrations with a time delay in culturing cells of 12, 24, 48 and 96 h. Additional measurements were performed on irradiated and blank blood samples with the aim to detect any increase in α and β activity after fission neutron irradiation. No difference was found. Results were compared to theoretically calculated values of the α and β activity released from natural radioactive isotopes. Result and Conclusion: As a conclusion it is shown that in our experimental conditions the secondary effects resulting from nuclear transformations of natural or induced radioactive isotopes, recoil reactions and accompanying α, β, and γ radiation are not the reason for the increase observed in chromosomal aberration yield in blood samples cultured with a time delay of at least 24 hours. (orig.)
Abstract (German)
Ziel: Um Differenzen in der Chromosomaberration zu erkennen, wurden weitere Experimente durchgefuehrt. Material und Methode: Es wurden zwei Kulturen angelegt: Unmittelbar nach der Bestrahlung mit Spaltneutronen und 96 Stunden nach der Bestrahlung. Die Proben von menschlichem peripherem Blut wurden in einem Mischfeld aus Neutronen-/Gammastrahlen fuer 1800 s bestrahlt und die biologischen Strahleneffekte mit Hilfe der instabilen Chromosomaberration nach einer Lagerung von 12, 24, 48 und 96 Stunden untersucht. Messungen an bestrahltem und nichtbestrahltem Blut zeigten keinen Anstieg der α- und β-Aktivitaeten der natuerlichen Isotopen verglichen. Ergebnis und Schlussfolgerung: Das Ergebnis der Experimente zeigte, dass Sekundaereffekte, die aus der Kernumwandlung von natuerlichen oder induzierten radioaktiven Stoffen resultieren, und die begleitende α-, β- und γ-Strahlung nicht die Ursache fuer das beobachtete Ansteigen der Chromosomaberration in Blutproben, die nach mindestens 24 Stunden kultiviert wurden, sind. (orig.)Additional details
Publishing Information
- Journal Title
- Strahlentherapie und Onkologie
- Journal Volume
- 173
- Journal Issue
- 2
- Journal Page Range
- p. 91-97.
- ISSN
- 0179-7158
- CODEN
- STONE4
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 28052874
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; CHROMOSOMAL ABERRATIONS; LYMPHOCYTES; URANIUM 235
- Descriptors DEC
- ACTINIDE NUCLEI; ALPHA DECAY RADIOISOTOPES; ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; EVEN-ODD NUCLEI; HEAVY NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LEUKOCYTES; MATERIALS; MINUTES LIVING RADIOISOTOPES; MUTATIONS; NUCLEI; RADIATION EFFECTS; RADIOISOTOPES; SOMATIC CELLS; SPONTANEOUS FISSION RADIOISOTOPES; URANIUM ISOTOPES; YEARS LIVING RADIOISOTOPES