Radiation sensitizing effect of chloroxoquinoline on Lewis lung cancer cells and xenograft tumors in mice

Objective: To study whether Chloroxoquinoline could enhance the radiation sensitivity of Lewis lung cancer cells and xenograft tumors in tumor-bearing mice. Methods: Both cell and animal experiments were divided into control group,drug group,irradiation group and combination group. MTT assay was used to detect the optical density of Lewis lung cancer cells cultured in vitro. The model of tumor-bearing mice was established first and then the tumor diameters of different groups were measured to figure out the tumor control rates and tumor growth delay. After irradiation,the blood samples in each group were collected to detect the amount of blood cells. Results: MTT showed 24 hours after irradiation,the cell survival fraction of combination group was lower than that of irradiation group, but no statistical differences (P>0.05). However, 48 hours later, the cell survival fraction of combination group was obviously lower than irradiation group with statistically significant differences (t=3.22, 4.23, 4.46, 4.58, P<0.05). Animal experiments showed the tumor growth curve of combination group was significantly lower than irradiation group. The tumor control rate and tumor growth delay of combination group was more above the other groups (t=3.15-3.59, P<0.05). Additionally, according to the analysis of blood samples, the hematological toxicity of chloroxoquinoline on mice was not found. Conclusion: Chloroxoquinoline shows its radiation sensitizing effect on Lewis lung cancer cells and xenograft tumors in mice without hematological toxicity. (authors)