Detection of coliform organisms in drinking water by radiometric method

The radiometric method has been used for detection of coliform bacteria in water. The method is based on measuring the released metabolic /Sup 14/CO/sub 2/from 14 C-lactose in growth media containing coliform organisms incubated at 37 deg. C. under continues shaking. This rapid and sensitive radiometric method permits the detection of even single coliform organisms within 6 hours in incubation. Using this automated method, a total of 102 samples (in duplicate) collected from different areas in and around Rawalpindi and Islamabad were assessed for coliform bacteria. Of these 102 samples, 50 were tap water samples, 40 from wells and 6 each were from Rawal and Simly dams. About 47% and 67% tap water samples, while 62% and 74% well water samples were found unsatisfactory from around Islamabad and Rawalpindi areas respectively, about 83% and 66% water samples from Rawal dam and Simly dam respectively were found to be unsatisfactory. (author)