Published April 1, 1983
| Version v1
Journal article
Enzyme-linked immunosorbent assay for leucine and methionine enkephalins
Description
An enzyme-linked immunosorbent assay for enkephalins was developed by coupling the peptides to a carrier molecule (bovine serum albumin) in order to allow the antibody-antigen reaction to take place in the solid phase. The assay was shown to be highly reproducible. Its sensitivity was 14 nmol/liter for leucine enkephalin and 27 nmol/liter for methionine enkephalin, which is similar to that obtained when the same antibodies were used in radioimmunoassay
Additional details
Publishing Information
- Journal Title
- Anal. Biochem.
- Journal Volume
- 130
- Journal Issue
- 1
- Series
- Anal. Biochem.
- Journal Page Range
- 83-87
- ISSN
- 0003-2697
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 15013390
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ANTIGEN-ANTIBODY REACTIONS; CHEMISORPTION; ENKEPHALINS; ENZYMES; LEUCINE; METHIONINE; QUALITATIVE CHEMICAL ANALYSIS; RADIOIMMUNOASSAY; SENSITIVITY
- Descriptors DEC
- AMINO ACIDS; CARBOXYLIC ACIDS; CHEMICAL ANALYSIS; CHEMICAL REACTIONS; ISOTOPE APPLICATIONS; LIPOTROPIC FACTORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PEPTIDES; POLYPEPTIDES; PROTEINS; SEPARATION PROCESSES; TRACER TECHNIQUES