Published July 1, 2022
| Version v1
Journal article
pH-activated DNA nanomachine for miRNA-21 imaging to accurately identify cancer cell
Creators
- 1. Xiangtan University. Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry (China)
- 2. Hunan Institute of Technology. School of Materials Science and Engineering (China)
Description
Abstract
MicroRNA (miRNA) imaging has been employed to distinguish cancer cells from normal cells by exploiting the overexpression of miRNA in cancer. Inspired by the acidic extracellular tumor microenvironment, we designed a pH-activated DNA nanomachine to enable the specific detection of cancer cells using miRNA imaging. The DNA nanomachine was engineered by assembling two hairpins (Y1 and Y2) onto the surface of a ZIF-8 metal-organic framework (MOF), which decomposed under acidic conditions to release the adsorbed DNA hairpin molecules in situ. The released hairpins were captured by the target miRNA-21 and underwent catalytic hairpin assembly amplification between Y1 and Y2. The detection limit for miRNA assays using the DNA nanomachine was determined to be 27 pM, which is low enough for sensitive detection in living cells. Living cell imaging of miRNA-21 further corroborated the application of the DNA nanomachine in the identification of cancer cell. Graphical abstract:Additional details
Identifiers
Publishing Information
- Journal Title
- Mikrochimica Acta
- Journal Volume
- 189
- Journal Issue
- 7
- Journal Page Range
- vp.
- ISSN
- 0026-3672
- CODEN
- MIACAQ
INIS
- Country of Publication
- Austria
- Country of Input or Organization
- Austria
- INIS RN
- 55014183
- Subject category
- S77: NANOSCIENCE AND NANOTECHNOLOGY; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
- Descriptors DEI
- DNA; IMIDAZOLES; NANOCHEMISTRY; NANOSTRUCTURES; NEOPLASMS; PH VALUE; RNA; TUMOR CELLS; ZIRCONIUM COMPOUNDS
- Descriptors DEC
- ANIMAL CELLS; AZOLES; CHEMISTRY; DISEASES; HETEROCYCLIC COMPOUNDS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; TRANSITION ELEMENT COMPOUNDS
Optional Information
- Copyright
- Copyright (c) 2022 © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022