Published November 2024 | Version v1
Journal article Metadata-only

Random and site-specific radiolabeling of [89Zr]Zr-DFO-anti-PD-L1-mAb iPET tracer

Creators

  • 1. China Medical University, Taichung, Taiwan (China). Department of Public Health
  • 2. Taichung Veterans General Hospital, Taichung, Taiwan (China). Department of Nuclear Medicine
  • 3. Central Taiwan University of Science and Technology, Taichung, Taiwan (China). Department of Medical Imaging and Radiological Sciences
  • 4. National Atomic Research Institute, Taoyuan, Taiwan (China). Department of Isotope Application Research
  • 5. National Dong Hwa University, Hualien, Taiwan (China). Department of Chemistry

Description

Zirconium-89 radiolabeled immuno-positron emission tomography (89Zr-iPET) has become a critical tool for patient stratification before and after immunotherapies for therapeutic evaluation. In this study, random and site-specific radiolabeling of 89Zr-iPET tracers were investigated. Traditional lysine-based conjugation and enzyme-based GlyCLICK® kit method were used to prepare DFO-anti-PD-L1-mAb conjugates with a chelator-to-antibody ratio (CAR) of 0-7 and 2.0, respectively. Then, conjugates with different CAR levels were radiolabeled with 89Zr to obtain randomly or site-specifically radiolabeled 89Zr-iPET tracer. (author)

Additional details

Publishing Information

Journal Title
Journal of Radioanalytical and Nuclear Chemistry
Journal Volume
333
Journal Issue
11
Journal Page Range
p. 5997-6005
ISSN
0236-5731
CODEN
JRNCDM

Conference

Title
11. International Conference on Isotopes and Expo
Acronym
11 ICI
Dates
23-27 Jul 2023
Place
Saskatoon, Saskatchewan (Canada)

INIS

Country of Publication
Hungary
Country of Input or Organization
Hungary
INIS RN
55103338
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Resource subtype / Literary indicator
Conference
Descriptors DEI
ANTIBODY FORMATION; CELL KILLING; CHELATING AGENTS; LABELLING; POSITRON COMPUTED TOMOGRAPHY; RECEPTORS; ZIRCONIUM 89
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; COMPUTERIZED TOMOGRAPHY; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; ELECTRON CAPTURE RADIOISOTOPES; EMISSION COMPUTED TOMOGRAPHY; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MEMBRANE PROTEINS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; TOMOGRAPHY; ZIRCONIUM ISOTOPES

Optional Information

Notes
31 refs.