In situ adsorption of bovine submaxillary mucin at the mica/aqueous solution interface
- 1. Laboratoire de Physico-Chimie des Surfaces et des Membranes, UER Biomedicale, Paris (France)
Description
An in situ adsorption measurement method at the mica/protein solution interface is described. An apparatus especially constructed for this purpose permits direct and continuous measurement of total adsorption (reversible and irreversible) of 14C-labelled proteins. Bovine submaxillary mucin (BSM), extracted from salivary glands, was acetylated with (CH314CO)2O. The results show the increase of BSM adsorbed on mica surfaces with its concentration in solution and adsorption time. Pseudo plateaux are obtained for all concentrations studied, indicating the formation of thick layers. The loosely bound fraction of adsorbed mucin is proportional to the bulk concentration in solution. The amount of BSM adsorbed increases in the neighbourhood of the isoelectric point of BSM (pH 3). (Auth.)
Additional details
Publishing Information
- Journal Title
- Colloids Surf.
- Journal Volume
- 9
- Journal Issue
- 4
- Series
- Colloids Surf.
- Journal Page Range
- 297-306
- ISSN
- 0166-6622
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 15067404
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ADSORPTION; AQUEOUS SOLUTIONS; CARBON 14 COMPOUNDS; DESORPTION; GLYCOPROTEINS; INTERFACES; LABELLED COMPOUNDS; MICA; SURFACES; TRACER TECHNIQUES
- Descriptors DEC
- CARBOHYDRATES; CARBON COMPOUNDS; DISPERSIONS; HOMOGENEOUS MIXTURES; ISOTOPE APPLICATIONS; MINERALS; MIXTURES; ORGANIC COMPOUNDS; PROTEINS; SACCHARIDES; SILICATE MINERALS; SOLUTIONS