Published May 2014 | Version v1
Journal article Metadata-only

X-ray subletal irradiation of murine 3T3 fibroblasts for feeder-layer

Creators

  • 1. Instituto Tecnologico de Costa Rica, Escuela de Biologia (Costa Rica)
  • 2. Instituto Tecnologico de Costa Rica, Escuela de Biologia, Centro de Investigacion en Biotecnologia (Costa Rica)
  • 3. Instituto Tecnologico de Costa Rica, Escuela de Ciencia e Ingenieria de los Materiales (Costa Rica)

Description

Keratinocytes, the main cells of the epidermis, require mitotically inactive but live feeder-layer cells for their survival and proliferation. Four X-ray irradiation doses on 3T3 murine fibroblasts were evaluated for feeder-layer production. Each dose effect was evaluated by cell counting and cellular viability determination (MTT), cell adhesion (plating efficiency) and cell migration (scratch wound healing). Mitotic arrest was suggested by nuclear staining (GIEMSA). An 80 Gy X-ray dose generated enough cell damage to cause mitotic arrest yet keeping metabolically active cells up to 25 days. (author)

Availability note (English)

Also available from Biblioteca Luis Demetrio Tinoco, Universidad de Costa Rica; Also available on line: https://doi.org/10.18845/tm.v28i5.2216

Abstract (Spanish)

Los queratinocitos, las principales celulas de la epidermis, requieren una capa alimentadora de celulas viables mitoticamente inactivadas para su supervivencia y proliferacion in vitro. Cuatro dosis de radiacion con rayos X en fibroblastos murinos 3T3 fueron evaluados para la produccion de capas alimentadoras. Los efectos de las dosis fueron evaluados mediante recuentos celulares y determinacion de la viabilidad (MTT), adhesion (eficiencia de plateo) y migracion celular (scratch wound healing). Ademas, las evidencias encontradas han sugerido el arresto mitotico mediante una tincion nuclear (GIEMSA). Una dosis de radiacion observada correspondiente a 80 Gy genero danos suficientes en la linea celular para causar el arresto mitotico, manteniendo a la vez la actividad metabolica basal durante 25 dias. (autor)

Additional details

Additional titles

Original title (Spanish)
Irradiacion subletal de fibroblastos murinos 3T3 con rayos x para su utilización como capa celular alimentadora

Identifiers

DOI
10.18845/tm.v28i5.2216;

Publishing Information

Journal Title
Tecnologia en Marcha
Journal Issue
special issue
Journal Page Range
p. 15-26
ISSN
0379-3982

INIS

Country of Publication
Costa Rica
Country of Input or Organization
Costa Rica
INIS RN
50007501
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Numerical Data
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; DOSE-RESPONSE RELATIONSHIPS; EPIDERMIS; EXPERIMENTAL DATA; FIBROBLASTS; RADIATION DOSES; SUBLETHAL IRRADIATION; X RADIATION
Descriptors DEC
ANIMAL CELLS; ANIMAL TISSUES; BIOLOGICAL EFFECTS; BODY; CONNECTIVE TISSUE CELLS; DATA; DOSES; ELECTROMAGNETIC RADIATION; EPITHELIUM; INFORMATION; IONIZING RADIATIONS; IRRADIATION; NUMERICAL DATA; ORGANS; RADIATION EFFECTS; RADIATIONS; SKIN; SOMATIC CELLS

Optional Information

Notes
Figs., refs.