Published January 1986
| Version v1
Journal article
In vivo dephosphorylation of WR-2721 monitored by 31P NMR spectroscopy
- 1. Armed Forces Radiobiology Research Institute, Bethesda, MD
Description
The in vivo dephosphorylation of the radioprotective agent S-2-[3-(aminopropylamino)]ethylphosphorothioic acid (WR-2721) in male CD2F1 mice was measured by 31P NMR spectroscopy after i.p. injection. The disappearance of the WR-2721 phosphate NMR signal with time was concurrent with an increase and splitting of the inorganic phosphate NMR signal. The more acidic inorganic phosphate resonance is shown to be attributed to phosphate (inorganic phosphate) in the urine. Using regression first-order kinetic analysis of data, after i.p. injection of 600 mg/kg, the half-life of WR-2721 was determined to be 40.9 +/- 5.9 (S.D.) min (n = 10)
Additional details
Publishing Information
- Journal Title
- J. Pharmacol. Exp. Ther.
- Journal Issue
- no.1
- Series
- J. Pharmacol. Exp. Ther.
- ISSN
- 0022-3565
- CODEN
- JPETA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 17068302
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BIOCHEMICAL REACTION KINETICS; IN VIVO; METABOLISM; MICE; NUCLEAR MAGNETIC RESONANCE; PHOSPHATES; PHOSPHORUS 31; PHOSPHORYLATION; RADIOPROTECTIVE SUBSTANCES; URINE
- Descriptors DEC
- ANIMALS; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CHEMICAL REACTIONS; DRUGS; ISOTOPES; KINETICS; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; REACTION KINETICS; RESONANCE; RESPONSE MODIFYING FACTORS; RODENTS; STABLE ISOTOPES; VERTEBRATES; WASTES