Structural analysis of pectin, polygalacturonic acid and pectinase enzyme iyophilysed

Pectic substances are pectinic acid, pectic acid and their salts. Pectin is a polysaccharide found in plants like fruits and vegetables etc in high level. Substances which have no methyl group are pectic acid and polygalacturonic acid. Pectic substances are heteropolysaccharides with 30,000-300,000 molecular weight. Pectic enzymes are known as enzyme destroying chain. Pectic enzyme's produce monogalacturonates by attaking the nonreducing end of the high molecular weight pectic acids. These are produced by saprophytic fungi, bacteria and some yeasts. They are used in fruit and vegetable technologies. In this research, crystal structures of pectin, polygalacturonic acid and Iyophilysed bacterial pectinase samples were studied by scanning electron microskop. Homogenous crystal structure was observed from the images at SEM. Pectin, polygalacturonic acid and pectinase enzyme was packed so compact and tightly that no transition of beam was observed. Pectin crystals have bigger size than polygalacturonic acid crystals. The crystals of substrata molecules was determined to be smaller than pectinase enzymes. Ca++ and Na++ cations are known to stimulate enzymatic activity. In second step of study, the elements which are thought to be present in the crystal structure of pectinase were analysed. Analysis results showed that Na, Zn, and Ca elements were found at concentrations of 60 %, 29.296 % and 6.555 %, respectively