Time-resolved X-ray diffraction of biological materials

Instrumental and specimen considerations pertinent to performing time-resolved x-ray diffraction on biological materials are discussed. Existing synchrotron x-ray sources, in conjunction with integrating x-ray detectors, have made millisecond diffraction experiments feasible; exposure times several orders of magnitude shorter than this will be possible with synchrotron sources now on the drawing boards. Experience grained from time-resolved studies together with order-of-magnitude estimates of specimen requirements can be used to determine the instrumental capabilities needed for various time-resolved experiments. Existing instrumental capabilities and methods of dealing with time-resolved specimens are reviewed