A source of error in the radioimmunoassay of α1 foeto-protein: the presence of bile in the serum
Creators
- 1. Centre National de la Recherche Scientifique, 94 - Villejuif (France). Inst. de Recherches Scientifiques sur le Cancer
Description
An attempt was made to find out whether bile could be responsible for false positives and for quantitative variations observed in the radioimmunoassay of α 1 foeto-protein. To this end a fixed amount of bile at different serum dilutions on the one hand, a variable quantity of bile at a given serum dilution on the other, were added to a positive (cord) serum and a negative (normal adult) serum. In the case of cord serum tested at 1/100, the value passes from 20000ng/ml without addition of bile to 60000, 300000 and 1300000ng/ml when tested at dilutions of 1/100, 1/1000 and 1/10000 respectively in the presence of the same quantity of bile. In the case of normal serum with a 6ng/ml content tested pure or diluted to 1/2, fractions of 110, 730 and 4500ng/ml respectively were found for 1/2, 1/5 and 1/10 dilutions with addition of a fixed amount of bile. The quantitative variations observed were dependent on the quantity of bile added: pure, diluted to 1/10, 1/100, 1/1000; they were large in the presence of pure or 1/10 diluted bile, almost non-existent with the same volume of bile diluted to 1/100 or 1/1000
Abstract (French)
On a tente de verifier si la bile pouvait etre responsable des faux positifs, ainsi que des variations quantitatives observees dans le dosage radio-immunologique d'α1 foeto-proteine. Pour ce faire on a ajoute a un serum positif (serum de cordon) et a un serum negatif (serum adulte normal), d'une part, une quantite fixe de bile a differentes dilutions de serum, d'autre part, une quantite variable de bile a une meme dilution de serum. Dans le cas du serum de cordon teste au 1/100, le taux passe de 20000ng/ml sans adjonction de bile a 60000, 300000 et 1300000ng/ml quand il est teste dilue respectivement au 1/100, 1/1000 et 1/10000 en presence de la meme quantite de bile. Dans le cas de serum normal dont le taux est de 6ng/ml teste pur ou au 1/2, on a trouve respectivement des taux de 110, 730 et 4500ng/ml pour des dilutions au 1/2, 1/5 et 1/10, dans lesquelles on a ajoute une quantite fixe de bile. Les variations quantitatives observees etaient fonction de la quantite de bile ajoutee: bile pure, diluee au 1/10, 1/100, 1/1000; elles etaient importantes en presence de bile pure ou diluee au 1/10, quasiment inexistantes avec un meme volume de bile diluee au 1/100, au 1/1000Additional details
Additional titles
- Original title (French)
- Une cause d'erreur dans le dosage radio-immunologique d'α1 foeto-proteine: la presence de bile dans le serum
Publishing Information
- ISBN
- 2727200013
- Imprint Title
- 17. French language symposiun on nuclear medicine. Paris, June 2-4, 1975
- Imprint Pagination
- p. 236-237.
- Report number
- CEA-CR--3
Conference
- Title
- 17. French language symposium on nuclear medicine.
- Dates
- 02 Jun 1975.
- Place
- Paris, France.
INIS
- Country of Publication
- France
- Country of Input or Organization
- France
- INIS RN
- 7236929
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- BILE; BLOOD SERUM; ERRORS; HEPATITIS; HEPATOMAS; PROTEINS; RADIOIMMUNOASSAY
- Descriptors DEC
- BIOLOGICAL MATERIALS; BODY FLUIDS; DIGESTIVE SYSTEM DISEASES; DISEASES; ISOTOPE APPLICATIONS; NEOPLASMS; ORGANIC COMPOUNDS; TRACER TECHNIQUES
Optional Information
- Notes
- Imprint:17. Colloque de medecine nucleaire de langue francaise. Paris, 2-4 juin 1975.;Rapports et communications.