Published November 15, 2006
| Version v1
Journal article
Neutron and X-ray reflectivity studies on DNA adsorption on mixed DPPC/DC-Cholesterol monolayers
- 1. Department of Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan (China)
- 2. National Synchrotron Radiation Research Center, Hsinchu 300, Taiwan (China)
- 3. Paul Scherrer Institut, CH-5232 Villigen-PSI (Switzerland)
Description
We have studied DNA adsorption on mixed DPPC/DC-Chol monolayers. Solid supported mixed monolayers on silicon wafers were prepared using Langmuir-Blodgett (LB) dipping technique. Neutron and X-ray reflectivity measurements were used to characterize these LB monofilms. For LB monofilms with DNA adsorption, the reflectivity data of the DPPC/DNA film are very close to that from the DPPC film, which indicates only minor DNA adsorption on the pure DPPC monolayer. Increasing the percentage of DC-Chol, film thickness increases. The DC-Chol/DNA film is thicker than the pure DC-Chol film (film thickness 18 A) by about 9 A due to the presence of adsorbed DNA. A model is presented to explain the structure of the lipid/DNA film
Additional details
Identifiers
- DOI
- 10.1016/j.physb.2006.05.121;
- PII
- S0921-4526(06)00948-3;
Publishing Information
- Journal Title
- Physica. B, Condensed Matter
- Journal Volume
- 385-386
- Journal Page Range
- p. 841-844
- ISSN
- 0921-4526
- CODEN
- PHYBE3
Conference
- Title
- 8. international conference on neutron scattering
- Dates
- 27 Nov - 2 Dec 2005
- Place
- Sydney (Australia)
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 38070825
- Subject category
- S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- ADSORPTION; CHOLESTEROL; DNA; FILMS; LIPIDS; NEUTRON DIFFRACTION; NEUTRONS; REFLECTIVITY; SILICON; SOLIDS; STRUCTURAL CHEMICAL ANALYSIS; THICKNESS; X RADIATION; X-RAY DIFFRACTION
- Descriptors DEC
- BARYONS; COHERENT SCATTERING; DIFFRACTION; DIMENSIONS; ELECTROMAGNETIC RADIATION; ELEMENTARY PARTICLES; ELEMENTS; FERMIONS; HADRONS; HYDROXY COMPOUNDS; IONIZING RADIATIONS; NUCLEIC ACIDS; NUCLEONS; OPTICAL PROPERTIES; ORGANIC COMPOUNDS; PHYSICAL PROPERTIES; RADIATIONS; SCATTERING; SEMIMETALS; SORPTION; STEROIDS; STEROLS; SURFACE PROPERTIES
Optional Information
- Copyright
- Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.