Purified lesion-recognizing enzyme to assay DNA repair in cultured animal cells

The in vitro enzymatic assay, which utilizes a purified uv endonuclease as a dimer probe, not only complements existing assays for DNA repair, but also lends itself to a number of novel studies. In fact, preliminary data (unpublished) indicate that this assay may be gainfully employed to elucidate repair processes operating on a variety of physicochemical alterations in DNA including those induced by uv and ionizing radiations, as well as various chemical agents displaying mutagenic, carcinogenic, or teratogenic activity