Studies of normal human osteoblasts and fibroblasts growth on composite functionalized β-type Ti₂₃Zr₂₅Nb (at%) dental implant materials containing 45S5 bioglass and silver

This study aims to present biological studies of ultrafine-grained Ti-based materials with niobium and zirconium content, and additionally modified with 45S5 bioglass and silver—Ti₂₃Zr₂₅Nb-9BG-Ag. Alloy and composites with 45S5 bioglass and silver were produced with the mechanical alloying approach. The in vitro cytocompatibility of these biomaterials was evaluated and compared with conventional microcrystalline titanium. During the studies, established cell lines of normal human periodontal ligament fibroblasts (HPdLF, CC-7049) and normal human osteoblasts (NHost, CC-2538) were cultured in the presence of tested materials, and their survival rate and proliferation activity were examined. Ti₂₃Zr₂₅Nb (at%) alloy and the composite with the addition of 9 wt% of 45S5 bioglass and doped with silver has been proven to be highly biocompatible based on the number of immunopositive NHost cells. Respectively, the composite non-doped with silver has been proven to be highly biocompatible based on the number of immunopositive HPdLF cells. Biocompatibility tests carried out indicate that the ultrafine-grained Ti₂₃Zr₂₅Nb-BG-Ag biomaterial can be a possible candidate for dental implant applications. (author)