Inhibition of T cell cytotoxicity by cyclosporine (CSA), adenosine (ADO), and an inhibitor of adenosine deaminase (ADA)

This paper tests the effect of cyclosporine (CSA), inhibitor EHNA and adenosine on cytolytic lymphoctyes (CTL). Cytotoxicity assay (5-hr 51Cr-release) was performed using anti-SV40-CTL as effectors in a ratio of 10:1 and 3:1 against the syngeneic target cells bearing SV40 antigens. At a higher E:T ratio of 10:1 there is a slight statistically significant reduction of cytotoxicity for all treatment groups except EHNA + CSA. The most reduction of cytolytic function is seen in treatment group where Ado, EHNA and CSA are combined