Published December 1976
| Version v1
Journal article
A 51Chromium release assay for phagocytic killing of Candida albicans
Creators
- 1. Saint Mary's Hospital Medical School, London (UK)
Description
Intracellular killing of Candida albicans was measured by a chromium release technique. Appropriate conditions were equal numbers of polymorphonuclear leucocytes (PMN) and 51Chromium labelled C. albicans (106/ml), fresh plasma at a final concentration of 2.5%, incubated at 37degC for 60 min. Using normal PMNs, 35-71% of releasable chromium was liberated into the supernatant under these conditions. This assay is easy to perform, requires a small amount of blood and offers an objective measurement of intracellular killing of C. albicans
Additional details
Identifiers
Publishing Information
- Journal Title
- Journal of Immunological Methods
- Journal Volume
- 13
- Journal Issue
- 3-4
- Series
- J. Immunol. Methods.
- Journal Page Range
- 227-233
- ISSN
- 0022-1759
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 8299146
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BLOOD PLASMA; CANDIDA; CHROMIUM 51; INTRACELLULAR DIGESTION; LABELLING; LEUKOCYTES; PHAGOCYTOSIS; RADIOASSAY; VIABILITY
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHROMIUM ISOTOPES; DIGESTION; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; FUNGI; INTERMEDIATE MASS NUCLEI; ISOTOPES; MICROORGANISMS; NUCLEI; PLANTS; RADIOISOTOPES; YEASTS
Optional Information
- Notes
- Updated automatically by Metadata and Full-Text Enrichment Agent