Effect of ligustrazine on the signal transduction of the recovery of bone marrow microenvironment in irradiation-injured mice
- 1. Huazhong Univ. of Science and Technology, Tongji Medical College, Wuhan (China). Tongji Hospital, Dept. of Hematology
Description
Objective: To evaluate the effect of Ligustrazine on bone marrow hematopoietic microenvi-ronment and the mechanism of signal transduction in radiation-injured mice. Methods: After irradiation by 6.0 Gy 60Co γ-ray, each mouse was given orally by 4 mg Ligustrazine twice a day for 13 days. On the 3th, 7th and 14th day, histopathologic sections from each paraffin-embedded ulnae were stained with anti-factor VIII-related antigen and anti-flk-1. The femurs were taken and the bone marrow nuclear cell suspension was made to culture bone marrow stromal cells (BMSCs), and the expression of phosphorylated FAK on BMSCs was assayed by Western blot. Results: The recovery of hematopoiesis in the Ligustrazine-treated group was sooner than the control group. The expression levels of flk-1 and phosphorylated FAK in the Ligustrazine-treated group were significant higher than the control group (P<0.05). Conclusion: The Ligustrazine can enhance the expression of flk-1 on bone marrow endothelial cells and the expression of phosphorylated FAK on BMSCs. This may be one of mechanisms of Ligustrazine to promote recovery of hematopoiesis after γ-irradiation
Additional details
Publishing Information
- Journal Title
- Chinese Journal of Radiological Medicine and Protection
- Journal Volume
- 23
- Journal Issue
- 2
- Journal Page Range
- p. 93-95
- ISSN
- 0254-5098
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 36021269
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL MODELS; BIOLOGICAL RECOVERY; BLOOD FORMATION; BONE MARROW; BONE MARROW CELLS; COBALT 60; DATA COVARIANCES; DOSE RATES; GAMMA RADIATION; HEMATOPOIETIC SYSTEM; MEDICINAL PLANTS; MICE; PHOSPHORYLATION; PHOSPHOTRANSFERASES; RADIATION INJURIES; STATISTICS; WHOLE-BODY IRRADIATION
- Descriptors DEC
- ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BODY; CHEMICAL REACTIONS; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; ENZYMES; EXTERNAL IRRADIATION; HEMATOPOIETIC SYSTEM; INJURIES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; IRRADIATION; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; MATHEMATICS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS-GROUP TRANSFERASES; PLANTS; PROTEINS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; TRANSFERASES; VERTEBRATES; YEARS LIVING RADIOISOTOPES