Water sources for apple trees in Alpine orchards: where does irrigation water go?

Understanding the dynamics and sources of root water uptake in agricultural systems is becoming increasingly important for implementing efficient and sustainable water resources management and, at the same time, for optimizing crop yield and quality under changing climatic conditions. In this work, we adopted the stable isotope approach to investigate the water sources accessed by apple trees in two orchards growing in the upper Etsch/Adige valley (South Tyrol, Eastern Italian Alps). We tested the general hypothesis that soil water, composed of a mixture of rain and irrigation water, was the main source for tree transpiration but that river water and groundwater mixed with soil water and contributed to root uptake for trees growing close to the river and with higher water table. Our results revealed that apple trees during the 2015 and 2016 growing seasons relied mostly on soil water present in the upper 20-40 cm of soils, with an apparently negligible contribution of groundwater and river water, irrespective of the field position across the valley bottom. The isotopic composition of xylem water did not reflect the one of irrigation water (and neither that of groundwater) but rather of rainfall and throughfall, as well as that of soil water. We related this "hidden" tracer signature of irrigation water to the effect of soil evaporation that strongly modified its original isotopic composition: irrigation and rain water infiltrated into the soil and mixed with isotopically fractionated soil water, and trees took up a mixture of water with different isotopic composition compared to the one of the original irrigation source. This work contributes to improve the understanding of water uptake strategies in Alpine apple orchards and paves the way for further analysis on the proportion of irrigation and rain water used by apple trees in mountain agroecosystems.