Protective effect of polysaccharides of tricholoma matsutake on hematopoietic function of X-rays irradiated mice

Objective: To explore the protective effect of the polysaccharides of tricholoma matsutake (PTM) on hematopoietic function of X-rays irradiated mice. Methods: Fifty ICR mice were divided into five groups randomly, 10 each group. The mice in normal control group (NC) and radiation control group (IC) were gaster-poured by 0.9% sodium chloride solution. The mice in PTM groups were gaster-poured by three different doses PTM (400, 800, 1200 mg·kg-1) for 7 d. The mice all groups except NC group were irradiated once with 2.0 Gy X-rays on the eighth day. The conony forming units-spleen (CFU-S), fibrolast colony forming unit (CFU-F), cell cycle of bone marrow cells and micronucleus rate of bone marrow potychromalic erythrocytes (PCE) were detected after radiation for 24 h. Results: Compared with IC group, the number of CFU-S in PTM groups decreased significantly (P<0.05 or P<0.01) and the number of CFU-F increased markedly (P<0.05 or P<0.01); in high and moderate doses PTM the micronucleus rates of bone marrow PCE and the percentage of bone marrow cells at G1 phase decreased significantly (P<0.05 or P<0.01); the percentage of cells at S phase increased significantly (P<0.05 or P<0.01). Conclusion: PTM has an obvious protective effect on hematopoietic function of X-rays irradiated mice. (authors)