Thin-layer ligand-exchange chromatography of cyclic nucleoside phosphates labelled with tritium, carbon-14 and phosphorus-33

The method for thin-layer ligand-exchange chromatography of the number of cyclic nucleoside-3', 5' phosphates was developed, the adsorbent being silicagel impregnated by copper (2)-ions. The conditions of the preparation of thin layers and the composition of solvent system containing ammonium hydroxide as the exchanging ligand were determined. The correlation of the mobility of the considered compounds with the stability of sorbing complexes was studied. The method of thin-layer ligand-exchange chromatography has been applied to the determination of the radiochemical purity of the considered compounds labelled with tritium, carbon-14, phosphorus-33