Published 1994 | Version v1
Miscellaneous Metadata-only

Purification of antibodies anti-interleukin-1 beta for radioimmunoassay

Creators

  • 1. Hospital das Clinicas, Campinas, SP (Brazil). Servico de Medicina Nuclear
  • 2. Saint Bartholomew's Hospital, London (United Kingdom)

Description

The sensitivity of a radioimmunoassay is dependent on the affinity of the antibody. The isolation of specific very high affinity antibodies is therefore a key requirement. In this project purified antibodies were prepared by affinity chromatography from polyclonal antisera anti-Interleukin-1 beta involving stepwise elution from pH 7.0 to 2.5 contributing to the development of a sensitive radioimmunoassay for measurement of human IL-1 beta. (author). 7 refs, 2 figs, 1 tab

Part of:
Proceedings of the 5. General Congress on Nuclear Energy. v. 3

Additional details

Additional titles

Original title (Portuguese)
Purificacao de anticorpos anti-intertleucina-1 beta para fins de radioimunoensaio

Publishing Information

Imprint Title
Proceedings of the 5. General Congress on Nuclear Energy. v. 3
Imprint Pagination
356 p.
Journal Page Range
p. 919-921.

Conference

Title
5. General Congress on Nuclear Energy.
Dates
28 Aug - 2 Sep 1994.
Place
Rio de Janeiro, RJ (Brazil).

INIS

Country of Publication
Brazil
Country of Input or Organization
Brazil
INIS RN
26056466
Subject category
S60: APPLIED LIFE SCIENCES; S62: RADIOLOGY AND NUCLEAR MEDICINE;
Resource subtype / Literary indicator
Conference, Non-conventional Literature
Descriptors DEI
ANTIBODIES; CHROMATOGRAPHY; IMMUNE SERUMS; LEUCINE; PURIFICATION; RADIOIMMUNOASSAY; SENSITIVITY
Descriptors DEC
AMINO ACIDS; CARBOXYLIC ACIDS; IMMUNOASSAY; ISOTOPE APPLICATIONS; ORGANIC ACIDS; ORGANIC COMPOUNDS; RADIOASSAY; SEPARATION PROCESSES; TRACER TECHNIQUES

Optional Information

Notes
Imprint:Anais do 5. Congresso Geral de Energia Nuclear. v. 3.