Studies on growth hormone: characterization of radioiodinated hormone and of receptors

Human growth hormone (hGH) has been prepared and characterised to a high specific activity with 125I suitable for use in competitive binding assays. The suitability of various methods for labelling hGH for use in radioimmuno - and radioreceptor assays is assessed. An investigation of the specificity of hGH receptors in the liver and mammary gland of pregnant rabbit is described and the effect of solubilisation on the specificity and affinity of membrane-band receptors from these areas is assessed. The ability of anti-body-bound hGH to react with membrane-bound receptors from rabbit liver and mammary gland was investigated. (U.K.)