Utilisation of Gamma Counter in Biodistribution Study by Cut and Count Approach

Full-text:Biodistribution (BQC) of 99mTc-DTPA in Sprague Dawley rats was evaluated in six (6) different time points within the range of 5 min to 24 h.Through cut and count approach, three (3) rats were used for each time point.The gamma radiation emitted from 99mTc isotope in 13 organs/samples was counted using Gamma Counter (Wizard2 Perkin Elmer, USA). Prior to use, the Gamma Counter shall undergo normalization, background measurement and Instrument Performance Assessment Measurement. Correction to the Gamma Counter efficiency was performed by applying radioisotope (99mTc) standard data to BQC data. This correction needs to be performed for every isotope used in BQC cut and count study. (author)