Published December 15, 1981 | Version v1
Journal article Metadata-only

A multivalent antibody radioimmunoassay (MARIA) for screening specific antibody secretion by lymphocyte hybridoma cultures

Creators

  • 1. National Inst. of Neurological Diseases and Stroke, Bethesda, MD (USA)

Description

A new radioimmunoassay which is suitable for screening lymphocyte hybridoma cultures for monoclonal antibody production is described. Solid-phase antigen is first incubated with the sample, and secondly with soluble 125I-labeled antigen. When the sample contains multivalent specific antibody, the labeled antigen will be bound to the solid-phase antigen. Because no anti-immunoglobulin reagent is used as a second reagent, this type of radioimmunoassay offers several advanatages, in particular for the production of monoclonal anti-idiotypic antibodies. (Auth.)

Additional details

Publishing Information

Journal Title
J. Immunol. Methods
Journal Volume
47
Journal Issue
2
Series
J. Immunol. Methods.
Journal Page Range
183-189
ISSN
0022-1759

INIS

Country of Publication
Netherlands
Country of Input or Organization
Netherlands
INIS RN
13671892
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ANTIGENS; IODINE 125; LABELLED COMPOUNDS; LYMPHOCYTES; NEOPLASMS; RADIOIMMUNOASSAY; SENSITIVITY; SPECIFICITY
Descriptors DEC
ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; NUCLEI; ODD-EVEN NUCLEI; RADIOISOTOPES; SOMATIC CELLS; TRACER TECHNIQUES