Published March 1990 | Version v1
Journal article

Identification and analysis of hydrogen uptake (HUP) genes of several associative nitrogen fixing bacteria with rice plant

  • 1. Chinese Academy of Agricultural Sciences, Beijing, BJ (China). Inst. for Application of Atomic Energy

Description

All of the tested species (strains) in this work can reduce TTC, suggesting that they contain hydrogen uptake hydrogenase. Hybridization with Rhizobium japonicum hup gene indicated that there was homology between restricted DNA and the probe for Alcaligenes faecalis A15, Enterobacter cloacae EnSs, Klebsiella planticola DWUL2 and Pseudomonas saccharophila. Negative results were obtained for E. cloacae E26 and K. oxytoca NG13. Hup genes of A. faecalis A15 were located on chromosomal DNA, however, it was located on the larger plasmid for E. cloacae EnSs. Nif gene and hup gene are located on the same replicon. Hup gene from different hup+ microorganisms was not homology inevitably

Additional details

Publishing Information

Journal Title
Acta Agriculturae Nucleatae Sinica
Journal Volume
4
Journal Issue
1
Series
Acta Agric. Nucleatae Sin.
Journal Page Range
19-24
ISSN
1000-8551
CODEN
HEXUE

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
22043293
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
AUTORADIOGRAPHY; AZOTOBACTER; DNA; ELECTROPHORESIS; GENES; HYBRIDIZATION; HYDROGENASES; PROBES; RICE
Descriptors DEC
BACTERIA; CEREALS; ENZYMES; GRAMINEAE; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; PLANTS