Published 1987
| Version v1
Book
Methods for hybridization and quantitation of mRNA in individual brain cells
Creators
- 1. Univ. of Arkansas for Medical Sciences, 4301 W. Markham, Little Rock, AR (USA)
Description
In situ hybridization techniques developed to quantitate the levels of specific messenger RNAs (mRNAs) relative to the levels of total polyadenylated (poly-A) mRNA are detailed here together with our protocols for combining immunohistochemical localization of proteins encoded by mRNAs that are localized by in situ hybridization. Specifically, the authors will demonstrate the use of the protocols they have used for in situ hybridization of cDNA, synthetic oligodeoxynucleotides, and RNA probes radiolabelled with either [/sup 3/H], [/sup 35/S], or [/sup 32/P], to paraffin-embedded mammalian tissue preserved with a variety of fixatives
Additional details
Publishing Information
- Publisher
- Oxford University Press.
- Imprint Place
- New York, NY (USA)
- Imprint Title
- In situ hybridization
- Journal Page Range
- p. 97-110.
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 20056266
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BRAIN; DIAGNOSIS; DNA; DNA-CLONING; GENETIC MAPPING; HYBRIDIZATION; LABELLING; MESSENGER-RNA; NERVE CELLS; PHOSPHORUS 32; PROTEINS; RADIOIMMUNOASSAY; RADIOPHARMACEUTICALS; SPECIFICITY; SULFUR 35; TRITIUM
- Descriptors DEC
- ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; CLONING; DAYS LIVING RADIOISOTOPES; DRUGS; EVEN-ODD NUCLEI; HYDROGEN ISOTOPES; IMMUNOASSAY; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; MATERIALS; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; RADIOACTIVE MATERIALS; RADIOISOTOPES; RNA; SOMATIC CELLS; SULFUR ISOTOPES; TRACER TECHNIQUES; YEARS LIVING RADIOISOTOPES