Feasibility Study on RI Application to a Biochip

Microarrays have recently become a precious research tool for proteomics and clinical investigation. Their applications to the diagnosis of a disease have emerged as a significant promise for medical advances. In this study, we report on an efficient strategy for the detection of phosphorylation of a substrate catalysed by kinase, using radioisotope detection technique for a protein bio chip. This technique does not employ the use of the blocking step which is commonly used in conventional methods to prevent non-specific binding. It was found that the usage of a radioisotope detection technique has the advantages of being highly sensitive and time saving when compared to other conventional methods. The results can be applied when using radioisotope detection technique to develop bio chips to determine the activity of a protein kinase. Further, it can be a useful tool for a high throughput screening and for studying protein-protein interactions