Published January 2003 | Version v1
Journal article Metadata-only

Study on trace elements distribution in rabbit metallothioneins by synchrotron radiation X-ray fluorescence analysis

Creators

  • 1. Chinese Academy of Sciences, Beijing (China). Inst. of High Energy Physics, Laboratory of Nuclear Analytical Techniques
  • 2. Chinese Academy of Sciences Beijing (China). Inst. of High Energy Physics, National Laboratory of Synchrotron Radiation

Description

The distributions of metal ions in rabbit metallothionein (MT) and its protein band in sodium dodecyl sulfate-polyacrylamide gel after electrophoresis were determined by synchrotron radiation X-ray fluorescence (SRXRF) analysis. Results showed that MT-1 mostly contained zinc, which amounted to 97.9% of the total metallic content and MT-2 mainly contained zinc and cadmium. This paper also introduced the method to determine the relative amounts of protein-bound metal ions in situ by combination of electrophoretic separation and SRXRF analysis

Additional details

Publishing Information

Journal Title
Nuclear Techniques
Journal Volume
26
Journal Issue
1
Journal Page Range
p. 17-20
ISSN
0253-3219

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
35052373
Subject category
S60: APPLIED LIFE SCIENCES; S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
CADMIUM; CONCENTRATION RATIO; ELECTROPHORESIS; METALLOTHIONEIN; PROTEINS; RABBITS; SYNCHROTRON RADIATION; TRACE AMOUNTS; X-RAY FLUORESCENCE ANALYSIS; ZINC
Descriptors DEC
ANIMALS; BREMSSTRAHLUNG; CHEMICAL ANALYSIS; ELECTROMAGNETIC RADIATION; ELEMENTS; MAMMALS; METALLOPROTEINS; METALS; NONDESTRUCTIVE ANALYSIS; ORGANIC COMPOUNDS; PROTEINS; RADIATIONS; VERTEBRATES; X-RAY EMISSION ANALYSIS