Published 1992 | Version v1
Book Metadata-only

Preparation and characterization of reagents for direct radioimmunoassay of cortisol

Creators

  • 1. National Lab. of Engineering and Industrial Technology, Sacavem (Portugal). Dept. of Radioisotopes

Description

Preparation and characterization of reagents suitable for direct radioimmunoassay (RIA) of cortisol in serum are described. Cortisol-3l(carboxymethyl) oxime-125iodohistamine (CCMO-Hist-125I) was purified by high pressure liquid chromatography (HPLC) and it specific activity determined. Coupling of antibodies to microcrystalline cellulose was performed and association/dissociation rates for the complex antigen antibody were evaluated. A suitable antibody was used to design a direct and accurate RIA for cortisol in serum. (author). 5 refs, 4 figs, 2 tabs

Part of:
Developments in radioimmunoassay and related procedures

Additional details

Publishing Information

Publisher
IAEA.
Imprint Place
Vienna (Austria)
ISBN
92-0-000392-3
Imprint Title
Developments in radioimmunoassay and related procedures
Imprint Pagination
601 p.
Series
Proceedings series.
Journal Page Range
p. 105-116.
ISSN
0074-1884

Conference

Title
Perspectives in developing countries.
Acronym
International symposium on radioimmunoassay and related procedures
Dates
26-30 Aug 1991.
Place
Vienna (Austria).

INIS

Country of Publication
Austria
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
23058067
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Conference, Numerical Data
Descriptors DEI
ACCURACY; BLOOD SERUM; EXPERIMENTAL DATA; HYDROCORTISONE; IODINE 125; LIQUID COLUMN CHROMATOGRAPHY; RADIOIMMUNOASSAY; SENSITIVITY; ULTRAVIOLET SPECTRA
Descriptors DEC
ADRENAL HORMONES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; CHROMATOGRAPHY; CORTICOSTEROIDS; DATA; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; GLUCOCORTICOIDS; HORMONES; HYDROXY COMPOUNDS; IMMUNOASSAY; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KETONES; MATERIALS; NUCLEI; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PREGNANES; RADIOASSAY; RADIOISOTOPES; SEPARATION PROCESSES; SPECTRA; STEROID HORMONES; STEROIDS; TRACER TECHNIQUES

Optional Information

Secondary number(s)
IAEA-SM--324/57.