Published August 2009 | Version v1
Report Metadata-only

Cytometric assessment of histone H2AX phosphorylation in human melanoma cells irradiated with carbon ions

Creators

  • 1. Inst. of Modern Physics, Chinese Academy of Sciences, Lanzhou (China)
  • 2. National Inst. of Radiological Sciences, Chiba, Chiba (Japan)

Description

The aim of this study is to investigate kinetic variation of histone H2AX phosphorylation (γH2AX) in human melanoma cells (OCM-1) after irradiation with carbon ions. We analyzed cell survival, cell cycle distribution and gamma H2AX immunofluorescence change using flow cytometry. Results suggested that γH2AX positive cells increased as a function of radiation dose (0.5-4 Gy) followed by dose-dependent decay. In this study, we concluded that there was significant linear correlation between surviving fraction and residual γH2AX level in human melanoma OCM-1 cells 8 hours after carbon ion irradiations. (author)

Part of:
Proceedings of NIRS-IMP joint symposium on carbon ion therapy

Additional details

Identifiers

URL
http://id.nii.ac.jp/1657/00073726/;

Publishing Information

Imprint Title
Proceedings of NIRS-IMP joint symposium on carbon ion therapy
Imprint Pagination
194 p.
Journal Page Range
p. 128-133
Report number
NIRS-M--225

Conference

Title
NIRS-IMP joint symposium on carbon ion therapy
Dates
14-15 Aug 2009
Place
Lanzhou (China)

INIS

Country of Publication
Japan
Country of Input or Organization
Japan
INIS RN
53066814
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Resource subtype / Literary indicator
Conference, Non-conventional Literature
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; CARBON IONS; CELL CYCLE; CELL FLOW SYSTEMS; HEAVY IONS; HIMAC ACCELERATOR; MELANOMAS; PHOSPHORYLATION; RADIATION DOSES; TUMOR CELLS
Descriptors DEC
ACCELERATORS; ANIMAL CELLS; BIOLOGICAL EFFECTS; CARCINOMAS; CHARGED PARTICLES; CHEMICAL REACTIONS; CYCLIC ACCELERATORS; DISEASES; DOSES; EPITHELIOMAS; HEAVY ION ACCELERATORS; IONS; NEOPLASMS; RADIATION EFFECTS; SYNCHROTRONS

Optional Information

Notes
This record replaces 44074409