Effect of 60Co γ radiation on the valyl-tRNA synthetase isolated from chick embryo brain
Creators
- 1. Orszagos Frederic Joliot-Curie Sugarbiologiai es Sugaregeszsegugyi Kutato Intezet, Budapest (Hungary)
Description
he effect of 60Coγ irradiation on the activity of valyl-tRNA synthetase isolated from chick embryo brain was studied. The enzyme activity exponentially decreased in the dose range 10 to 200 krad. The first step of the enzyme action, i.e. amino acid activation, was found to be less sensitive to irradiation than the whole reaction, the formation of valyl-tRNA. 2-Mercapto ethanol and/or glycerol had a significant radioprotective effect. The lesion caused by radiation in the enzyme was also influenced by its concentration during exposure (diluted effect). According to gel-electrophoretic experiments, no chain rupture occurred in the enzyme molecule. Not even a change in Ksub(m) was observed; however, the maximum velocity of the reaction was found to decrease with increasing radiation dose. (author)
Additional details
Publishing Information
- Journal Title
- Int. J. Radiat. Biol. Relat. Stud. Phys., Chem. Med.
- Journal Volume
- 34
- Journal Issue
- 4
- Series
- Int. J. Radiat. Biol. Relat. Stud. Phys., Chem. Med.
- Journal Page Range
- 329-336
- ISSN
- 0020-7616
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- United Kingdom
- INIS RN
- 10444901
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- BIOCHEMICAL REACTION KINETICS; BRAIN; CATALYSIS; CHEMICAL RADIATION EFFECTS; DOSE-RESPONSE RELATIONSHIPS; EXPERIMENTAL DATA; GAMMA RADIATION; GRAPHS; IN VITRO; LIGASES; METABOLISM; QUANTITY RATIO; RADIOSENSITIVITY; TRANSFER RNA; VALINE
- Descriptors DEC
- AMINO ACIDS; BODY; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; DATA; DATA FORMS; ELECTROMAGNETIC RADIATION; ENZYMES; INFORMATION; IONIZING RADIATIONS; KINETICS; NERVOUS SYSTEM; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; RADIATION EFFECTS; RADIATIONS; REACTION KINETICS; RNA