Published September 1, 1981 | Version v1
Journal article Metadata-only

Efficient method for in vitro labeling proteins to high specific radioactivity using 3H-NaBH4 in Dimethylformamide

Creators

  • 1. Univ. of Southern California, Los Angeles

Description

A method for labeling proteins or insoluble protein matrices to high specific radioactivity using 3H-NaBH4 in anhydrous dimethylformamide without the presence of formaldehyde or other aidehydes and ketones is presented. Chemical analysis of the labeled proteins suggests that the labeling mechanism involves methylation of lysyl residues. The procedure is simple and the labeled protein products retain their size and charge characteristics

Additional details

Publishing Information

Journal Title
Anal. Biochem.
Journal Volume
116
Journal Issue
1
Series
Anal. Biochem.
Journal Page Range
69-74
ISSN
0003-2697

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
13671490
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Resource subtype / Literary indicator
Numerical Data
Descriptors DEI
AMINO ACIDS; BIOASSAY; BIOLOGICAL PATHWAYS; BIOSYNTHESIS; CATALYSIS; CHEMICAL ACTIVATION; ENZYMES; EXPERIMENTAL DATA; KIDNEYS; MEASURING METHODS; METABOLISM; METHYL RADICALS; RATS; SCINTISCANNING; TRITIUM COMPOUNDS
Descriptors DEC
ALKYL RADICALS; ANIMALS; BODY; CARBOXYLIC ACIDS; COUNTING TECHNIQUES; DATA; DIAGNOSTIC TECHNIQUES; HYDROGEN COMPOUNDS; INFORMATION; MAMMALS; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PERFORMANCE TESTING; RADICALS; RADIOISOTOPE SCANNING; RODENTS; SYNTHESIS; TESTING; VERTEBRATES