Published May 2000
| Version v1
Journal article
Imaging Tc-99m-labeled FGF-1 targeting in rats
Description
Recombinant human acidic fibroblast growth factor (FGF-1) was radiolabeled with 99mTc by the HYNIC method. The 99mTc-FGF-1 retained its representative molecular mass, heparin affinity, cellular binding to both low (Kd=9.5 nM) and high (Kd=125 pM) affinity sites, and mitogenic activity. Gamma camera imaging after intravenous dosing in rats confirmed high liver and kidney binding. Heparin significantly decreased 99mTc-FGF-1 liver uptake and increased urinary excretion. These studies illustrate a new method for imaging FGF-1 targeting under various conditions
Additional details
Identifiers
- PII
- S0969805100000901;
Publishing Information
- Journal Title
- Nuclear Medicine and Biology
- Journal Volume
- 27
- Journal Issue
- 4
- Journal Page Range
- p. 407-414
- ISSN
- 0969-8051
- CODEN
- NMBIEO
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- Brazil
- INIS RN
- 33035969
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- FIBROBLASTS; GAMMA CAMERAS; GROWTH FACTORS; HEPARIN; HYDRAZINE; IMAGE PROCESSING; LABELLED COMPOUNDS; RADIONUCLIDE KINETICS; RATS; TECHNETIUM 99; UPTAKE
- Descriptors DEC
- AMINES; ANIMAL CELLS; ANIMALS; ANTICOAGULANTS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CAMERAS; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; DRUGS; HEMATOLOGIC AGENTS; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; KINETICS; MAMMALS; MITOGENS; MUCOPOLYSACCHARIDES; NITROGEN COMPOUNDS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; POLYSACCHARIDES; PROCESSING; PROTEINS; RADIOISOTOPES; RODENTS; SACCHARIDES; SOMATIC CELLS; TECHNETIUM ISOTOPES; VERTEBRATES; YEARS LIVING RADIOISOTOPES
Optional Information
- Copyright
- Copyright (c) 2000 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.