Evaluation of salbutamol in pure form and pharmaceutical formulations using spectrophotometry and green nonionic surfactant of cloud point extraction

The purpose of the study was to identify a quick, simple and sensitive spectrophotometric technique for the assessment of salbutamol (SAL). The proposed approach depends on SAL's azo-coupling reaction with BEN. The orange color of the reaction product was analyzed at λ_max (480 nm) against a blank solution. It was obeyed to beer-lambert show over concentration between (2.5-17.5 mg.L⁻¹) with LOD (1.283 mg.L⁻¹), LOQ (4.234 mg.L⁻¹) and molar absorptivity (12922.74 L.mol⁻¹.cm⁻¹). The procedure revealed high sensitivity for assessment of chosen drug. The new cloud point extraction technique was also successfully utilized for the extraction of pharmaceutically pure SAL drug, (also known as 2-[4-(2,4,4-trimethylpentan-2-yl) phenoxy] ethanol), was selected as green extraction solvent due to its properties and structure. The influence of various parameters, such as the kind and volume of surfactant, salt, temperature and incubation time on the CPE of salbutamol was investigated in detail and a set of perfect conditions was established. A correlation coefficient (R²) was 0.9914 for the calibration curve was obtained. The LOD, LOQ and molar absorptivity were 0.041 mg.L⁻¹, 0.12 mg.L⁻¹ and 47024.61 L.mol⁻¹.cm⁻¹, respectively. We believe that our suggested method is rapid, very convenient, and cost effective for the determination of salbutamol drug in the various samples. (paper)