Retention characteristic of ranitidine hydrochloride on new polymer-based in zwitter ion chromatography-hydrophilic interaction chromatography stationary phases

Two zwitterionic stationary phases with largely corroborated capacities were obtained by attachment sulfobetaine monomers (H/sub 2/C=CHC/sub 6/H/sub 4/CH/sub 2/N+(CH/sub 3/)2-CH/sub 2/-SO/sub 3/- and H/sub 2/C=CHC/sub 6/H/sub 4/CH/sub 2/N+(CH/sub 3/)2-(CH/sub 2/)5-SO/sub 3/-) onto a PS/DVB particles were investigated for chromatographic separation of ranitidine hydrochloride. The different chain lengths are used as an investigative tool for the retention behavior of pharmaceutical ranitidine hydrochloride. The retention behavior of ranitidine hydrochloride was examined with eluent at various ACN contain, buffer concentrations and pH. The separation mechanism is based on partitioning in reversed phase and ZIC ion exchange resulting in a mixed mode for the ranitidine hydrochloride. A direct calibration graph was constructed for ZIC1 and ZIC5 columns and it was found that the linear range (10-1000 ng.ml-1), RSD% (0.41-1.55), LOD (1.55 and 2.56 ng.ml-1), LOQ (5.17 and 8.53 ng.ml/sup -1/), Recovery% (101.06 ± 1.15 and 100.47 ± 0.12) and E/sub rel/% (1.666 ± 0.78 and 0.47 ± 0.12), respectively. (author)