Ion microscopy analysis of the intracellular distribution of 14C-or 15N-labelled cytotoxic drugs

This study has concerned the localization, by the means of ion microscopy of human tumoral cells (in culture), of two synthesized cytostatic compounds: BD40 or Pazelliptine and Intoplicine. These two active compounds (cancer research) have been labelled either with 15N on the terminal amino dialkyl group of the lateral chain, either, for Intoplicine, with 14C on the carbon 3 of the aromatic cycle. These two drugs are considered as poisons for topo-isomerases, and they were used to localize these cellular multiplication key-enzymes. Unfortunately, pazelliptine and intoplicine specificity appeared much too low. However, encouraging results were obtained, particularly on 15N-labelling utilization. 4 figs., 9 refs