Published November 1, 2012 | Version v1
Journal article Metadata-only

Nanoflow electrospinning serial femtosecond crystallography

Creators

  • 1. SLAC National Accelerator Laboratory, Menlo Park, CA 94025 (United States)
  • 2. Lawrence Berkeley National Laboratory, Berkeley, CA 94720 (United States)
  • 3. Technische Universität Berlin, Strasse des 17 Juni 135, 10623 Berlin (Germany)
  • 4. Stockholm University, S-106 91 Stockholm (Sweden)
  • 5. Stanford University, Stanford, CA 94025 (United States)
  • 6. European Synchrotron Radiation Facility, Grenoble (France)
  • 7. Umeå Universitet, Umeå (Sweden)

Description

A low flow rate liquid microjet method for delivery of hydrated protein crystals to X-ray lasers is presented. Linac Coherent Light Source data demonstrates serial femtosecond protein crystallography with micrograms, a reduction of sample consumption by orders of magnitude. An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min−1 to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min−1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption

Availability note (English)

Available from http://dx.doi.org/10.1107/S0907444912038152; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478121

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478121;
DOI
10.1107/S0907444912038152;
PII
S0907444912038152;

Publishing Information

Journal Title
Acta Crystallographica. Section D: Biological Crystallography
Journal Volume
68
Journal Issue
Pt 11
Journal Page Range
p. 1584-1587
ISSN
0907-4449
CODEN
ABCRE6

INIS

Country of Publication
Denmark
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46057605
Subject category
S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
Descriptors DEI
CRYSTALLOGRAPHY; CRYSTALS; DELIVERY; DIFFRACTION; FLOW RATE; LIGHT SOURCES; LINEAR ACCELERATORS; LIQUIDS; PROTEINS; REDUCTION; RESOLUTION; SPIN; SUSPENSIONS; X-RAY LASERS
Descriptors DEC
ACCELERATORS; ANGULAR MOMENTUM; CHEMICAL REACTIONS; COHERENT SCATTERING; DISPERSIONS; FLUIDS; LASERS; ORGANIC COMPOUNDS; PARTICLE PROPERTIES; RADIATION SOURCES; SCATTERING

Optional Information

Copyright
Copyright (c) International Union of Crystallography 2012
Notes
PMCID: PMC3478121; PMID: 23090408; PUBLISHER-ID: lv5021; OAI: oai:pubmedcentral.nih.gov:3478121