Published 1983 | Version v1
Journal article Metadata-only

Electron radiation damage in thin specimens prepared for X-ray microanalysis by cryoultramicrotomy, freeze substitution and normal resin embedding

Creators

  • 1. Tenovus Institute for Cancer Research, Welsh National School of Medicine, Heath Park, Cardiff, UK

Description

Analysis of biological tissue is ultimately limited by the transformation of elemental composition under the influence of the electron beam. Specimen erosion depends on elemental composition and molecular structure, analytical conditions and specimen temperature. Studies reported here indicate a variability in elemental and mass loss for various preparations of thin sections. With careful choice of specimen preparation techniques and analytical operating conditions, mass and element losses from the specimen can be minimised

Additional details

Publishing Information

Journal Title
Scanning Electron Microsc.
Journal Issue
p. 2001-2018
Series
Scanning Electron Microsc.
ISSN
0586-5581

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
15063882
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
CHEMICAL RADIATION EFFECTS; ELECTRON BEAMS; ELECTRON MICROPROBE ANALYSIS; LOSSES; SAMPLE PREPARATION; TISSUES
Descriptors DEC
BEAMS; BODY; CHEMICAL ANALYSIS; LEPTON BEAMS; MICROANALYSIS; NONDESTRUCTIVE ANALYSIS; PARTICLE BEAMS; RADIATION EFFECTS