Published January 1992 | Version v1
Journal article Metadata-only

Elemental imaging of cartilage by scanning x-ray microscopy

Creators

  • 1. Department of Physics, King's College, Strand, London WC2R 2LS (United Kingdom)
  • 2. Institute of Orthopaedics (University of London), Royal National Orthopaedic Hospital, Stanmore, Middlesex (United Kingdom)
  • 3. Department of Physics, State University of New York, Stony Brook, New York 11794 (United States)
  • 4. Center for Advanced Radiation Sources, University of Chicago, Chicago, Illinois 60637 (United States)

Description

Elemental imaging via scanning transmission x-ray microscopy (STXM) and scanning fluorescence x-ray microscopy (SFXM) has been used to image calcium deposits in cartilage. In the case of STXM, 0.1 μm thick sections were imaged to investigate the proximity of calcium deposits in relation to chondrocyte cells. The resolution available was 0.5 μm, and field widths of up to 25 μm were used at this resolution. The resolution available in SFXM was 10 μm, and field widths of up to 2 mm were used at this resolution on 5-μm thick specimens. Together these techniques were used to map calcium deposits at the cellular level, and at the full tissue size level

Additional details

Publishing Information

Journal Title
Review of Scientific Instruments
Journal Volume
63
Journal Issue
1
Series
Rev. Sci. Instrum.
Journal Page Range
588-590
ISSN
0034-6748
CODEN
RSINA

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
23059873
Subject category
S46: INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL RADIATION EFFECTS; BIOMEDICAL RADIOGRAPHY; CARTILAGE; DESIGN; IMAGES; MICROSCOPES; MICROSCOPY; NSLS; RESOLUTION; X RADIATION
Descriptors DEC
BIOLOGICAL EFFECTS; BODY; CONNECTIVE TISSUE; DIAGNOSTIC TECHNIQUES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; MEDICINE; RADIATION EFFECTS; RADIATION SOURCES; RADIATIONS; SYNCHROTRON RADIATION SOURCES; TISSUES