Novel Ag@TiO₂ nanocomposite synthesized by electrochemically active biofilm for nonenzymatic hydrogen peroxide sensor

A novel nonenzymatic sensor for H₂O₂ was developed based on an Ag@TiO₂ nanocomposite synthesized using a simple and cost effective approach with an electrochemically active biofilm. The optical, structural, morphological and electrochemical properties of the as-prepared Ag@TiO₂ nanocomposite were examined by UV–vis spectroscopy, X-ray diffraction, transmission electron microscopy and cyclic voltammetry (CV). The Ag@TiO₂ nanocomposite was fabricated on a glassy carbon electrode (GCE) and their electrochemical performance was analyzed by CV, differential pulse voltammetry and electrochemical impedance spectroscopy. The Ag@TiO₂ nanocomposite modified GCE (Ag@TiO₂/GCE) displayed excellent performance towards H₂O₂ sensing at − 0.73 V in the linear response range from 0.83 μM to 43.3 μM, within a detection limit and sensitivity of 0.83 μM and ∼ 65.2328 ± 0.01 μAμM⁻¹ cm⁻², respectively. In addition, Ag@TiO₂/GCE exhibited good operational reproducibility and long term stability. - Graphical abstract: Synthesis of Ag@TiO₂ nanocomposite by electrochemically active biofilm for H₂O₂ sensing. - Highlights: • Electrochemically active biofilm (EAB) • EAB mediated synthesis of Ag@TiO₂ nanocomposite • Ag@TiO₂ nanocomposite modified glassy carbon electrode • Ag@TiO₂/GCE for H₂O₂ sensing • Nonenzymatic sensor for H₂O₂