Estimation of gluconeogenesis and glucose utilization in carbohydate deficient growing rats
Description
A carbohydrate deficient diet based on food grade oleic acid and soybean oil and a minimally adequate level of casein protein was supplemented with graded levels of glucose (0, 4, 10, 65%), and casein protein (12% basal level plus 4, 6, 20%). Weanling rats were fed the respective diets for 28 days. Under anesthesia in fed state, the right jugular vein and left carotid artery were cannulated. NaH14CO3 and 3H-glucose labelled on C6 were injected into aorta via carotid and blood samples taken from vena cava via jugular over a period of 30 minutes. Rate of increase of blood 14C-glucose was the indicator of gluconeogenesis (GLNG). Disappearance of blood 3H-glucose was the measure of glucose flux. Relative rate of GLNG was very high in basal unsupplemented rats, and glucose flux was very low. Rats growing rapidly with minimum supplementation (4% glucose or 6% casein) showed the lowest relative rate of GLNG and maximum glucose flux, of the order of 10 mg min-1 kg-1. GLNG increased with higher levels of glucose and casein, but flux did not increase. The fed state glucose flux extrapolated to 24 hour basis was approximately 2X greater than the dietary intake of glucose and its equivalent of glucogenic precursors in rats fed the basal diet and low levels of supplements. Adjustment for lower flux in post absorptive state, based on flux in fasted rats, reduced the differences between observed flux and intake
Additional details
Publishing Information
- Journal Title
- Fed. Proc., Fed. Am. Soc. Exp. Biol.
- Journal Volume
- 45
- Journal Issue
- 4
- Journal Series
- Fed. Proc., Fed. Am. Soc. Exp. Biol.
- Journal Page Range
- 976
- ISSN
- 0014-9446
- CODEN
- FEPRA
Conference
- Title
- 70. annual meeting of the Federation of American Society for Experimental Biology.
- Dates
- 13-18 Apr 1986.
- Place
- St. Louis, MO (USA).
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18036278
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Conference
- Quality check status
- Yes
- Descriptors DEI
- BLOOD; CARBOHYDRATES; CARBON 14 COMPOUNDS; GLUCOSE; METABOLISM; NUTRITIONAL DEFICIENCY; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS;
- Descriptors DEC
- ALDEHYDES; ANIMALS; BIOLOGICAL MATERIALS; BODY FLUIDS; CARBON COMPOUNDS; HEXOSES; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; MATERIALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; RODENTS; SACCHARIDES; VERTEBRATES;
Optional Information
- Secondary number(s)
- CONF-8604222--.