Field trial of a brucellosis competitive enzyme linked immunoabsorbent assay (ELISA)
Creators
- 1. INTA-CICV Instituto Patobiologia Area Bacteriologia, Buenos Aires (Argentina)
Description
The purpose of this study was to evaluate the performance of a competitive ELISA system for the diagnosis of bovine brucellosis in comparison to conventional aerological tests routinely used in Argentina. A total of 2.500 serum samples, comprising Brucella-free herds, vaccinated cattle and naturally infected animals, was tested by the following tests: buffered plate agglutination, Rose Bengal, 2-mercaptoethanol, complement fixation, and indirect and competitive ELISAs. Specificity and relative sensitivity at each test were determined. The competitive ELISA was considered suitable for detection of vaccinated animals and had higher specificity than the other tests. The results point to the potential use of the test as a complementary assay in the brucellosis control programme in Argentina. (author)
Files
29067687.pdf
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Additional details
Publishing Information
- Imprint Title
- Diagnosis and epidemiology of animal diseases in Latin America. Proceedings of the final research co-ordination meetings of FAO/IAEA/SIDA co-ordinated research projects
- Imprint Pagination
- 302 p.
- Journal Page Range
- p. 163-167
- ISSN
- 1011-4289
- Report number
- IAEA-TECDOC--1055
Conference
- Title
- Final research co-ordination meeting on the use of ELISA for epidemiology and control of foot and mouth disease and bovine brucellosis in Latin America
- Dates
- 14-18 Apr 1997
- Place
- Vienna (Austria)
INIS
- Country of Publication
- International Atomic Energy Agency (IAEA)
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 29067687
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Conference
- Descriptors DEI
- BACTERIAL DISEASES; BLOOD SERUM; BRUCELLA; CATTLE; COMPARATIVE EVALUATIONS; DIAGNOSIS; DIAGNOSTIC TECHNIQUES; ENZYME IMMUNOASSAY; SENSITIVITY; SPECIFICITY; VACCINES; VETERINARY MEDICINE
- Descriptors DEC
- ANIMALS; BACTERIA; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; DISEASES; DOMESTIC ANIMALS; EVALUATION; IMMUNOASSAY; INFECTIOUS DISEASES; MAMMALS; MATERIALS; MEDICINE; MICROORGANISMS; RUMINANTS; VERTEBRATES
Optional Information
- Notes
- 10 refs, 5 tabs