Published January 11, 2010 | Version v1
Journal article Metadata-only

DNA damage and mitochondria dysfunction in cell apoptosis induced by nonthermal air plasma

Creators

  • 1. Department of Electronic and Electrical Engineering, Pohang University of Science and Technology, Pohang 790-784 (Korea, Republic of)
  • 2. Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784 (Korea, Republic of)
  • 3. Department of Integrative Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784 (Korea, Republic of)

Description

Nonthermal plasma is known to induce animal cell death but the mechanism is not yet clear. Here, cellular and biochemical regulation of cell apoptosis is demonstrated for plasma treated cells. Surface type nonthermal air plasma triggered apoptosis of B16F10 mouse melanoma cancer cells causing DNA damage and mitochondria dysfunction. Plasma treatment activated caspase-3, apoptosis executioner. The plasma treated cells also accumulated gamma-H2A.X, marker for DNA double strand breaks, and p53 tumor suppressor gene as a response to DNA damage. Interestingly, cytochrome C was released from mitochondria and its membrane potential was changed significantly.

Additional details

Identifiers

DOI
10.1063/1.3292206;

Publishing Information

Journal Title
Applied Physics Letters
Journal Volume
96
Journal Issue
2
Journal Page Range
p. 021502-021502.3
ISSN
0003-6951
CODEN
APPLAB

INIS

Country of Publication
United States
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
41078433
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
AIR; ANIMAL CELLS; APOPTOSIS; BIOCHEMISTRY; BIOPHYSICS; DNA; GENES; MELANOMAS; MEMBRANES; MICE; MITOCHONDRIA; PLASMA; RADIOTHERAPY; STRAND BREAKS
Descriptors DEC
ANIMALS; CARCINOMAS; CELL CONSTITUENTS; CHEMISTRY; DISEASES; DNA DAMAGES; EPITHELIOMAS; FLUIDS; GASES; MAMMALS; MEDICINE; NEOPLASMS; NUCLEAR MEDICINE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHYSICS; RADIOLOGY; RODENTS; THERAPY; VERTEBRATES

Optional Information

Notes
(c) 2010 American Institute of Physics