Published July 30, 1982 | Version v1
Miscellaneous Restricted

Influence of some radioprotective and radiosensitizing compounds on the replicative and repair induced DNA synthesis of rats spleen cells in vitro

Creators

Description

The effect of cysteine, dithiothreitol, N-ethylmaleimide, cytosinearabinoside, ethidiumbromide, bleomycine and diethyldithiocarbamate on the replicative and repair induced DNA synthesis in vitro was tested by using rats spleen cells. Besides the incorporation of a labeled DNA precursor (TdR-3H) the sedimentation of DNA in sucrose gradients was inquired. With respect to the DNA synthesis an uniform mechanism of action for the radioprotective substances can't be seen. Thymocytes and spleen cells seem to possess different systems of repair; this may be an explanation for their different sensibility against ionizing radiation. (orig./MG)

Availability note (English)

MF available from INIS under the Report Number.

Abstract (German)

An Rattenmilzzellen wurde die Wirkung von Cystein, Dithiothreitol, N-Aethylmaleimid, Cytosinarabinosid, Ethidiumbromid und Diaethyldithiocarbamat auf die replikative und reparative DNA-Synthese in vitro untersucht. Neben dem Einbau einer radioaktiv markierten DNA-Vorstufe (TdR-3H) wurde die Sedimentation der DNA im Saccharosegradienten getestet. Unter Beruecksichtigung der DNA-Synthese ist ein einheitlicher Wirkungsmechanismus fuer radioprotektive Substanzen nicht zu erkennen. Thymus- und Milzzellen scheinen mit verschiedenen Reparatursystemen ausgestattet zu sein, was ihre unterschiedliche Empfindlichkeit gegenueber ionisierenden Strahlen und UV-Licht erklaeren koennte. (orig./MG)

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Additional details

Additional titles

Original title (German)
Einfluss einiger radioprotektiver und radiosensibilisierender Verbindungen auf die semikonservative und reparaturbedingte DNA-Synthese von Rattenmilzzellen in vitro

Publishing Information

Imprint Pagination
164 p.
Report number
INIS-mf--10690

INIS

Country of Publication
Germany
Country of Input or Organization
Germany
INIS RN
18073242
Subject category
S61: RADIATION PROTECTION AND DOSIMETRY;
Resource subtype / Literary indicator
Thesis
Descriptors DEI
ANIMAL CELLS; ANTINEOPLASTIC DRUGS; BIOCHEMICAL REACTION KINETICS; BLEOMYCIN; CYSTEINE; DNA REPAIR; DNA REPLICATION; EFFICIENCY; IN VITRO; RADIOPROTECTIVE SUBSTANCES; RADIOSENSITIZERS; RATS; SPLEEN; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS
Descriptors DEC
AMINO ACIDS; ANIMALS; ANTIBIOTICS; ANTIMITOTIC DRUGS; AZINES; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BODY; CARBOXYLIC ACIDS; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; PYRIMIDINES; REACTION KINETICS; RESPONSE MODIFYING FACTORS; RIBOSIDES; RODENTS; THIOLS; VERTEBRATES