Published 1976
| Version v1
Book
Thyroid-stimulating hormone (TSH), ch. 9
Creators
- 1. Harbor General Hospital, Torrance, Calif. (USA)
- 2. Pennsylvania Univ., Philadelphia (USA)
Description
A radioimmunoassay for TSH is described. Antisera produced in rabbits were examined by determining the dose response curve for TSH and the immunologically and chemically similar LH, FSH and HCG. By preabsorption with the most disturbing hormone, HCG, a specific antiserum was made. TSH was labelled with 131I or 125I by the chloramine-T method of Greenwood and purified by sephadex gel filtration. After incubation the bound and free labelled hormones were separated by the double antibody technique. Plasma values were obtained from 0.4-2 ng/ml and a rise in the TSH concentration was observed in newborn infants shortly after delivery
Additional details
Publishing Information
- Publisher
- North-Holland.
- Imprint Place
- Amsterdam, Netherlands
- ISBN
- 0 7204 0630 7
- Imprint Title
- Methods in radioimmunoassay of peptide hormones
- Imprint Pagination
- p. 103-110.
INIS
- Country of Publication
- Netherlands
- Country of Input or Organization
- Netherlands
- INIS RN
- 8301301
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- CHROMATOGRAPHY; IMMUNE SERUMS; INCUBATION; IODINE 125; IODINE 131; LABELLING; ORGANIC IODINE COMPOUNDS; PRECIPITATION; RADIOIMMUNOASSAY; SPECIFICITY; TSH
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; RADIOISOTOPES; SEPARATION PROCESSES; TRACER TECHNIQUES
Optional Information
- Notes
- Imprint:Reprinted from Methods in Investigative and Diagnostic Endocrinology, vols. 2A and 2B, 1973, North-Holland.