Published October 2007
| Version v1
Journal article
Characteristic properties of lipase from cowpea (vigna unguiculata) seedlings
Creators
- 1. Sindh Agriculture Univ., Tando Jam (Pakistan) Dept. of Physiology
- 2. University of Sindh, Jamshoro (Pakistan). Dept. of Biotechnology and Genetic Engineering
Description
Lipase activity was assayed in 4 days old cowpea seedlings using olive oil emulsion stabilized with 10% gum acacia as a substrate. The maximum lipase activity was observed at pH 8.0 with Tris-HCI buffer and at 30 degree C. The pH stability was found in between 7.5-8.5. Lipase activity was fairly stable up to 60 degree C and retaining 80% activity whereas, 26% lipase activity was remaining at 100 degree C within 15 minutes. Lipase activity was slightly increased in the presence of MnC/sub 2/ and decreased by the addition of Triton X 100, Tween 80, ZnCI/sub 2/ and mercaptoethanol. (author)
Additional details
Publishing Information
- Journal Title
- Science International (Lahore)
- Journal Volume
- 19
- Journal Issue
- 4
- Journal Page Range
- p. 295-298
- ISSN
- 1013-5316
INIS
- Country of Publication
- Pakistan
- Country of Input or Organization
- Pakistan
- INIS RN
- 39035715
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ENZYMES; GERMINATION; LIPASES; OLIVE OIL; PH VALUE; PLANT TISSUES; SEEDLINGS; VIGNA; ZINC CHLORIDES
- Descriptors DEC
- CARBOXYLESTERASES; CHLORIDES; CHLORINE COMPOUNDS; ENZYMES; ESTERASES; ESTERS; HALIDES; HALOGEN COMPOUNDS; HYDROLASES; LEGUMINOSAE; LIPIDS; MAGNOLIOPHYTA; MAGNOLIOPSIDA; OILS; ORGANIC COMPOUNDS; OTHER ORGANIC COMPOUNDS; PLANTS; PROTEINS; TRIGLYCERIDES; VEGETABLE OILS; ZINC COMPOUNDS; ZINC HALIDES