Constructing a novel Nanodevice powered by δ-free FoF1-ATPase
Creators
- 1. National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
- 2. Graduate School of the Chinese Academy of Sciences, Beijing (China)
Description
A Nanodevice was constructed by δ-free FoF1-ATPase within chromatophores and actin filaments through biotinlipid-streptavidin-biotin-(AC5)2Sulfo-OSu system. One actin filament linking with many chromatophores functions as the Nanodevice body and many δ-free FoF1-ATPase as the Nanodevice motors. Movement of the Nanodevice was observed directly by fluorescence microscopy with CCD camera after illumination. The moving speed was about 2.17-24.43 μm/s for various length Nanodevices and most of them were stopped by adding CCCP. This means that the Nanodevice was driven by PMF (proton-motive force) in the cooperating δ-free FoF1-ATPase. From bioengineering point of view, the cooperation of FoF1-ATPase is a very important research field in the future
Additional details
Identifiers
- DOI
- 10.1016/j.bbrc.2006.09.152;
- PII
- S0006-291X(06)02201-7;
Publishing Information
- Journal Title
- Biochemical and Biophysical Research Communications
- Journal Volume
- 350
- Journal Issue
- 4
- Journal Page Range
- p. 1013-1018
- ISSN
- 0006-291X
- CODEN
- BBRCA9
INIS
- Country of Publication
- United States
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 38027591
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ACTIN; BIOTIN; FILAMENTS; FLUORESCENCE; ILLUMINANCE; MICROSCOPY; NANOSTRUCTURES
- Descriptors DEC
- AZOLES; CARBOXYLIC ACIDS; EMISSION; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; IMIDAZOLES; LUMINESCENCE; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PHOTON EMISSION; PROTEINS; VITAMIN B GROUP; VITAMINS
Optional Information
- Copyright
- Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.